In order to ensure the biological activity and quality of the sample, the sample needs to be pre-frozen first, and pre-freezing in a refrigerator or a Benchtop Freeze Dryers to be placed on the shelf of the freeze-drying bin to carry out the main drying process of the sample. At this time, the moisture in the sample is directly sublimated into steam by the low temperature and high vacuum of the Benchtop Freeze Dryers, and is captured by the low temperature cold trap and the vacuum pump. After dozens of hours of main drying, most of the moisture of the sample is sublimated, and the sample is completely retained, and the sublimated gas is condensed into ice in the cold trap. Due to the pressure, gas is sublimated and then The cold trap is condensed into ice. When the ice is sublimated, it absorbs heat, causing the temperature of the sample itself to drop and slowing down the sublimation speed. In order to increase the sublimation speed and shorten the drying time, the product must be properly heated. In order to ensure the higher purity of the sample, the sample can be heated and lyophilized at this time, or the post-drying process can be performed. The sample temperature can reach room temperature, and all lyophilization processes are completed.
Advantages of working principle of Benchtop Freeze Dryers
1 The substance is always in a low temperature (freezing state) before drying, and the ice crystals are evenly distributed in the substance, and the sublimation process does not cause concentration due to dehydration, thereby avoiding side effects such as foaming and oxidation caused by water vapor.
2 The dry material is dry sponge with a porous volume, which is basically unchanged, without shrinkage, and is very soluble in water and returns to the form before lyophilization.
3 The principle of ordinary drying is to use heating, but these drying methods are carried out at temperatures above 0 ° C or higher, and the dried products vary greatly in physical and chemical shapes. In the process of freeze-drying, the growth of microorganisms and the action of enzymes cannot be carried out, and the original biological and chemical properties of dry substances are prevented from being largely prevented by Zui, and the stability is good, and it is easy to store for a long time.
4 The product is dried, desorbed, and tamponed in a sterile state in the freeze-drying chamber.
5 can remove 95% to 99% of the water in the substance.
6 It is especially suitable for the dry preservation of heat sensitive substances such as vaccines, strains, poisonous species, blood products and the like.
7 The main components of the Benchtop Freeze Dryers have excellent structure layout, which is conducive to transportation, installation and production operations; it is conducive to cleaning and maintenance, and meets GMP requirements.
In order to ensure the biological activity and quality of the sample, the sample needs to be pre-frozen first, and pre-freezing in a refrigerator or a Benchtop Freeze Dryers to be placed on the shelf of the freeze-drying bin to carry out the main drying process of the sample. At this time, the moisture in the sample is directly sublimated into steam by the low temperature and high vacuum of the Benchtop Freeze Dryers, and is captured by the low temperature cold trap and the vacuum pump. After dozens of hours of main drying, most of the moisture of the sample is sublimated, and the sample is completely retained, and the sublimated gas is condensed into ice in the cold trap. Due to the pressure, gas is sublimated and then The cold trap is condensed into ice. When the ice is sublimated, it absorbs heat, causing the temperature of the sample itself to drop and slowing down the sublimation speed. In order to increase the sublimation speed and shorten the drying time, the product must be properly heated. In order to ensure the higher purity of the sample, the sample can be heated and lyophilized at this time, or the post-drying process can be performed. The sample temperature can reach room temperature, and all lyophilization processes are completed.
Advantages of working principle of Benchtop Freeze Dryers
1 The substance is always in a low temperature (freezing state) before drying, and the ice crystals are evenly distributed in the substance, and the sublimation process does not cause concentration due to dehydration, thereby avoiding side effects such as foaming and oxidation caused by water vapor.
2 The dry material is dry sponge with a porous volume, which is basically unchanged, without shrinkage, and is very soluble in water and returns to the form before lyophilization.
3 The principle of ordinary drying is to use heating, but these drying methods are carried out at temperatures above 0 ° C or higher, and the dried products vary greatly in physical and chemical shapes. In the process of freeze-drying, the growth of microorganisms and the action of enzymes cannot be carried out, and the original biological and chemical properties of dry substances are prevented from being largely prevented by Zui, and the stability is good, and it is easy to store for a long time.
4 The product is dried, desorbed, and tamponed in a sterile state in the freeze-drying chamber.
5 can remove 95% to 99% of the water in the substance.
6 It is especially suitable for the dry preservation of heat sensitive substances such as vaccines, strains, poisonous species, blood products and the like.
7 The main components of the Benchtop Freeze Dryers have excellent structure layout, which is conducive to transportation, installation and production operations; it is conducive to cleaning and maintenance, and meets GMP requirements.
How to use the Benchtop Freeze Dryers
1. Before using the Benchtop Freeze Dryers, place the prepared items in a low-temperature refrigerator or liquid nitrogen to freeze the contents completely before freezing.
2. The vacuum tube is connected between the main unit and the vacuum pump, and the international standard clamp is used at the joint. The clamp contains a sealing rubber ring. Before connecting, apply a proper amount of vacuum grease on the rubber ring and then clamp it with a clamp.
3. The power supply socket of the vacuum pump is installed on the right side of the main unit, and the power line of the vacuum pump is connected.
4. Check the vacuum pump and confirm that the vacuum pump oil has been filled. Do not run without oil. The oil level must not be lower than the oil mirror midline.
5. The “O” type sealing rubber ring above the host cold trap should be kept clean. When used for 4 times, a thin layer of vacuum grease can be applied. The plexiglass cover is placed on the rubber ring and gently rotated a few times. seal
Benchtop Freeze Dryers precautions
1. Do not switch frequently during the operation of the Benchtop Freeze Dryers. If the refrigerator stops running due to operational errors, it cannot be started immediately. Wait at least 20 minutes before starting again to avoid damage to the refrigerator.
2. After each freeze-drying, after the ice on the cold trap coil is turned into water, clean it with a towel and freeze it. When the "inflating valve" is opened and inflated into the cold trap, it must be slow to avoid damaging the vacuum gauge.
How does the Benchtop Freeze Dryers work?